The test kit Vitrotest® Ureaplasma-IgМ is an enzyme linked immunosorbent assay (ELISA) for the qualitative and semiquantitative determination of IgM class antibodies to Ureaplasma urealiticum in human serum or plasma.

Determination of IgM antibodies to Ureaplasma urealiticum in the test kit Vitrotest® Ureaplasma-IgМ is based on a solid phase, «IgM-capture» ELISA in a two-step incubation procedure.

○ ТК096 – 96 tests

  • Solid phase: breakable microplate ELISA is coated with monoclonal anti-IgM antibodies.
  • Conjugate: recombinant U. urealiticum antigen, conjugated to horseradish peroxidase.
  • Chromogen: ready to use TMB solution.
  • Volume of sample for analysis: 10 μl.
  • Assay time: 1h 30 min.

The microorganism Ureaplasma urealyticum causes inflammatory diseases of the human genitourinary system and Ureaplasma is often found in women with vaginitis and cystitis.

Penetration of the pathogen into the upper parts of the reproductive system can lead to the disruption of the reproductive functions. In men, U.urealyticum is the cause of nongonococcal urethritis and prostatitis (up to 50% of cases). The role of ureaplasmas in the development of most cases of urolithiasis has been proven. Often U.urealyticum leads to postpartum sepsis in women.

To diagnose ureaplasmosis, both direct methods for identifying ureaplasmas (polymerase chain reaction, immunofluorescence reaction, isolation of pure culture) and serological methods for detecting antibodies specific to Ureaplasma urealyticum are applied. Determination of antibodies in enzyme immunoassay is especially important in chronic ureaplasmosis, as well as in ascending ureaplasma infection. ELISA is a minimally invasive research method that allows for a comprehensive diagnosis of urogenital diseases.

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