The test kit Vitrotest HIV Ag/Ab is an enzyme linked immunosorbent assay (ELISA) for the simultaneous detection of HIV p24 antigen and total antibodies (IgG, IgA, IgM) to HIV1 (group M and O) and HIV2 in human serum or plasma.
Simultaneous detection of the HIV1 p24 core antigen and total antibodies to human immunodeficiency virus types 1 and 2 (HIV1/2) in the Vitrotest HIV Ab/Ag test kit is based on a solid-phase sandwich ELISA in a two-step incubation procedure.
○ TK132 – 96 tests
○ TK133 – 192 tests
○ TK134 – 480 tests
- Solid phase: breakable microplate ELISA is coated with monoclonal antibodies specific to p24 HIV1 and recombinant HIV 1/2 antigens.
- Conjugate: solution of streptavidin and recombinant HIV1/2 antigens conjugated to horseradish peroxidase.
- Chromogen: ready to use TMB solution.
- Volume of sample for analysis: 70 μl.
- Assay time: 60/30/15 min.
- Analytical sensitivity: 2 IU/ml.