The test kit Vitrotest Transglutaminase IgA is an enzyme linked immunosorbent assay (ELISA) for the quantitative determination of IgA class antibodies to tissue transglutaminase (human tTG) in human serum or plasma.
Determination of IgA class antibodies in the test kit Vitrotest Transglutaminase IgA is based on a solid phase, indirect ELISA in a two-step incubation procedure.
- TK156 – 96 tests
- Solid phase: strip ELISA plate pre-coated with recombinant human tissue transglutaminase 2.
- Conjugate: monoclonal antibodies to human IgA conjugated with horseradish peroxidase.
- Chromogen: ready to use TMB solution.
- Sample volume: 10 μl.
- Assay time: 1 h 15 min.
- Linearity range: 7 – 164 U/ml.
Celiac disease (gluten enteropathy) is a chronic immune-mediated disease that develops in genetically predisposed individuals in response to the ingestion of gluten, which is found predominantly in wheat, rye, and barley, with a global seroprevalence ranging from 0.5% to 2% of the general population (1). The disease can occur at any age and is characterized by inflammation of the small intestinal mucosa, villous atrophy, and impaired absorption of nutrients.
Tissue transglutaminase 2 plays a key role in the pathogenesis of celiac disease and is the major autoantigen associated with the production of characteristic celiac disease-related autoantibodies. Tissue transglutaminase is a calcium-dependent enzyme that modifies gluten peptides through deamidation, promoting activation of the gluten-specific T-cell immune response, followed by the development of conditions leading to inflammation and damage to the small intestinal mucosa.
IgA class antibodies to tissue transglutaminase (anti-tTG IgA) are one of the main serological markers of celiac disease and a recommended test for its initial laboratory screening and for monitoring the effectiveness of treatment in patients with a previously established diagnosis of celiac disease. IgG class antibodies to tTG may also be produced in celiac disease; however, their diagnostic value is lower compared with anti-tTG IgA. In patients with selective deficiency of total IgA, serological tests for the determination of IgG antibodies are used, in particular anti-tTG IgG or antibodies to deamidated gliadin peptides (DGP IgG).
Determination of IgA class antibodies to tissue transglutaminase by enzyme-linked immunosorbent assay (ELISA) is an important tool for the serological evaluation of patients with suspected celiac disease and individuals at increased risk of developing the disease, as ELISA is characterized by high diagnostic accuracy, convenience, and minimal invasiveness.