The Vitrotest® Total-IgG immunodiffusion kit is designed for the quantitative determination of total immunoglobulin G (IgG) in human serum or plasma by radial immunodiffusion in a Mancini technique.

The determination of IgG antibodies in the Vitrotest® Total-IgG immunodiffusion kit is based on an immunoprecipitation reaction. Immunoglobulins of the test sample diffuse in a semi-solid agarose gel containing immune polyclonal serum and form a precipitation ring when equivalent concentrations are reached. The diameter of the ring depends on the concentration of IgG. Comparison with the control allows to determine the concentration of immunoglobulins in the tested serum.

○ TK100 – 70 tests

  • Immunodiffusion medium – ready-to-use agar gel with wells containing highly specific to human IgG immune serum.
  • Control – is a mixture of purified human immunoglobulins of classes G, A, and M with known concentrations.
  • The volume of sample for analysis – 3 μL.
  • Assay time – 24 hours.


Immunoglobulins (antibodies) are a group of glycoproteins found in mammalian plasma and tissue fluid. Five classes of immunoglobulins are found in humans – IgG, IgA, IgM, IgD, and IgE – which differ in function, molecular size, charge, and carbohydrate content.

IgG is the main isotype of immunoglobulins in normal human serum, comprising for 70-75% of the total amount of serum immunoglobulins. Its concentration normally ranges from 6.0 to 16.0 g/L. Class G immunoglobulins are major class of antibodies of the secondary immune response, they are synthesized for a longer time after an antigenic stimulus and have the unique property of initiating anti- and pro-inflammatory reactions. Antibodies of this class bind also soluble antigens, so they are the main part of antitoxins. In humans, IgG is transported from mother to fetus across the placenta, creating passive immunity for the neonatal period. Excessive production of immunoglobulins G may be associated with an active infectious process, certain types of immunoproliferative diseases, or other conditions that lead to hyperstimulation of B-cell clones that produce IgG. IgG deficiency can be primary (congenital), which is rare, or secondary (acquired), caused by factors that suppress humoral immunity.

To determine the content of total human IgG in diagnostic laboratories, the radial immunodiffusion (RID) method is widely used, which is considered the standard for measuring of different classes immunoglobulins in human serum and plasma.