Specific IgE

«Vitrotest Specific-IgE» is intended for the quantitative determination of specific IgE class antibodies in human serum or plasma. Used with biotinylated allergens produced by Ramintek IPC.

Quantitative determination of allergen-specific IgE is based on the principle of «capture»-variant enzyme immunoassay (ELISA).

• Variant of configuration: 96-1T (12xS8-1T).
• Solid phase - ELISA plate coated with the first monoclonal antibodies specific for IgE.
• Calibrators - 0 IU/mL, 0.35 IU/ml, 1 IU/ml, 5 IU/ml, 25 IU/ml, 100 IU/ml.
• Biotinylated monoclonal anti-IgE antibodies or biotinylated allergens.
• Conjugate: streptavidin conjugated with horseradish peroxidase, binds to biotin in the complex formed in the solid phase.
• Chromogen: one-component TMB.
• Volume of serum for analysis: 50 mcl.
• Timing Analysis - 2 hours.